Composite

Part:BBa_K2448054:Design

Designed by: Ioana Popescu   Group: iGEM17_Evry_Paris-Saclay   (2018-12-27)


D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum with a C-terminal HisTag under the contr


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pTacI promoter (BBa_K864400) + RBS (BBa_K2448019) + D-psicose 3-epimerase from Clostridium cellulolyticum with a C-terminal HisTag coding sequence (BBa_K2448053)


Source

cloning

References